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Friday, November 13, 2020 | History

2 edition of Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen found in the catalog.

Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen

Scott G Huling

Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen

a laboratory and field study

by Scott G Huling

  • 257 Want to read
  • 35 Currently reading

Published by Robert S. Kerr Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency in Ada, Okla .
Written in English

    Subjects:
  • Hazardous waste site remediation -- Experiments,
  • Hydrogen peroxide -- Biodegradation -- Experiments

  • Edition Notes

    Statementby Scott G. Huling, Bert E. Bledsoe, and Mark V. White
    ContributionsBledsoe, Bert E, White, Mark V, Robert S. Kerr Environmental Research Laboratory
    The Physical Object
    Paginationviii, 48 p. :
    Number of Pages48
    ID Numbers
    Open LibraryOL13612465M

    This review describes a new means of control and stimulation of microorganisms involved in the bioremediation of sediments and waterlogged soils. This emerging technology is derived from sedimentary microbial fuel cells, and consists in ensuring aerobic respiration of aerobic microbial populations in anaerobic conditions by means of a fixed potential anode in order to evacuate the ?PaperID= We show that glycolate oxidase (GLO) physically interacts with catalase (CAT) in rice leaves, and that the interaction can be deregulated by salicylic acid. We propose that the physical association–dissociation of GLO and CAT, in response to environmental stress or stimuli, serves as a specific mechanism to modulate H2O2 levels in ://(16)


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Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen by Scott G Huling Download PDF EPUB FB2

United States Environmental Protection Agency Robert S. Kerr Environmental Research Laboratory Ada, OK Research and Development EPA//S/ Apr. Project Summary Enhanced Bioremediation Utilizing Hydrogen Peroxide as a Supplemental Source of Oxygen: A Laboratory and Field Study Scott G.

Huling, Bert E. Bledsoe, and Mark V. White Remedial actions at hazardous waste ?Dockey= Laboratory and field scale studies were conducted to investigate the feasibility of using hydrogen peroxide as a supplemental source of oxygen for bioremediation of an aviation gasoline fuel spill.

Field samples of aviation gasoline contaminated aquifer material were artificially enhanced with nutrients to promote microbiological degradation of fuel carbon in a laboratory column experiment. he  › EPA Home. EPA/// February ENHANCED BIOREMEDIATION UTILIZING HYDROGEN PEROXIDE as a SUPPLEMENTAL SOURCE Of OXYGEN: A LABORATORY AND FIELD STUDY by Scott G.

Ruling Bert E. Bledsoe Extramural Activities and Assistance Division Robert S. Kerr Environmental Research Laboratory U.S. Environmental Protection Agency Ada, Oklahoma ?Dockey= Get this from a library. Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen: a laboratory and field study.

[Scott G Huling; Bert E Bledsoe; Mark V White; Robert S. Kerr Environmental Research Laboratory.] Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen. Ada, Okla.: Robert S. Kerr Environmental Research Laboratory, Office of Research and Development, U.S.

Environmental Protection Agency, [] (OCoLC) Material Type: Government publication, National government publication, Internet resource   Hydrogen peroxide, which is commonly used as an oxygen source for in situ biodegradation, tends to decompose into water and oxygen gas. The rate of this decomposition relative to the oxygen demand of the contaminated aquifer is important to the success of an in situ process.

The objective of this study, which was performed at Eglin Air Force Base in northwest Florida, was to evaluate in situ   Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen: a laboratory and field study.

Bioremediation, EPA//S/, Google Scholar. Wilson JT, Armstrong JM, Rifai HS. A full-scale field demonstration on the use of hydrogen peroxide for in situ bioremediation of an aviation gasoline contaminated :// The hydrogen peroxide-amended groundwater is pumped into infiltration galleries or injection wells located in or near suspected source areas.

Generally, the infiltration/injection and groundwater extraction scheme is designed to promote the circulation and distribution of hydrogen peroxide and dissolved oxygen through the treatment area (EPA ).

Bioventing combines the capabilities of soil venting and enhanced bioremediation to cost-effectively remove light and middle distillate hydrocarbons from vadose zone soils and the groundwater table. Soil venting removes the more volatile fuel components from unsaturated soil and promotes aerobic biodegradation by driving large volumes of air into the ://   Enhanced bioremediation is an efficient technology when compared to alternative technologies.

The two major advantages are lower capital cost and the ability to perform the task insitu. Beginning aroundenhanced biodegradation, or biostimulation, became widely accepted as a leading treatment technology. Many effective biostimulation supplements have been demonstrated   hydrogen peroxide and dissolved oxygen through the treatment area.

Exhibit XII-5 provides a conceptual illustration of a hydrogen peroxide enhanced aerobic bioremediation system. The precipitation of chemical oxidants (e.g., iron oxides) can present potentially significant equipment fouling Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen: A laboratory and field study, EPA///, Kerr Environ.

Entrapment and mobilization of residual oil in bead packs, (). Entrapment, stability and persistence of air bubbles in soil water, The objectives of the laboratory study described in this paper were (1) to determine the effectiveness of four nutrient solutions and a control in stimulating the microbial degradation of toluene in the unsaturated zone as an alternative to bioremediation methodologies such as air sparging, in situ vitrification, or others (Part I), and (2) to compare the effectiveness of the addition of the Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen: A laboratory and field study.

Final report, August November Article   Bioventing is cheaper than use of nitrates or hydrogen peroxide as the source of electron acceptors. Land treatment: It is a full scale bioremediation technology in which contaminated soils, sediments or sludges are periodically turned over (tilled) and ?doi=rjet Enhanced bioremediation utilizing hydrogen per-oxide as a supplemental source of oxygen: a labo- Hydrogen peroxide trial tanks received two, mg/L H2O2 treatments 8 h apart, until hatch.

The Feasibility of Utilizing Hydrogen Peroxide as a Source of Oxygen in Bioremediation Effectiveness and Kinetics of Hydrogen Peroxide and Nitrate-Enhanced Biodegradation of Hydrocarbons Laboratory Evaluation of the Utilization of Hydrogen Peroxide for Enhanced Biological Treatment of Petroleum Hydrocarbon Contaminants in Soil Hydrogen peroxide, which was found to be a suitable alternative to pure oxygen as a source of oxygen for in-situ biodegradation, will also be used in the full-scale demonstration.

Based on the Moffett Field results, the plan at Edwards to demonstrate in-situ aerobic cometabolism of TCE is to pulse in a primary substrate, toluene, while   Enhanced Bioremediation Utilizing Hydrogen Peroxide as a Supplemental Source of Oxygen, EPA///; NTIS: PB Hunter, Marie, July " Biological Remediation of Contaminated Groundwater Systems, " Pollution :// Enhanced bioremediation utilizing hydrogen peroxide as a supplemental source of oxygen: a laboratory and field study.

Unpublished Report, US EPA, Ada, OK, 48 pp. 21 Hoag, G.E. and M.C. Marley. Gasoline residual saturation in unsaturated uniform aquifer materials.

://   A Case History of Enhanced Bioremediation Utilizing Pure Oxygen Injection p. 65 Studies of In Situ Bioremediation of an Aquifer Polluted by Toluene p.

71 Cycled Air Sparging: Field Results in a High-Velocity Aquifer p. 77 Biodegradation and Volatilization During IAS - A Push-Pull Test p.

Finally, another great source of information about Hydrogen Peroxide is the book 'The Truth about Food Grade Hydrogen Peroxide' by James Paul Roguski.

In it he recommends that 4 tablespoons (60ml) of 3% food grade hydrogen peroxide be added to every gallon of distilled water that you drink. Curt. Hydrogen Peroxide: Curse or Cure Dr. David Williams   Enhanced Bioremediation and Biosurfactant HC green sustainable technology, cleans, desorbs, reduces odors, and degrades fuels, oils, transformer oils, lubricants, chlorinated and non-chlorinated solvents.

HC degrades oil and solvents in soil, ballast, gravel, groundwater, surfacewater. HC used on over sites, HC is less toxic than EPA NCP chemical and   Enhanced Bioremediation Utilizing Hydrogen Peroxide as a Supplemental Source of Oxygen: A Laboratory and Field Study.

EPA//S/, NTIS PB/XAB In Situ Generation of Oxygen By Electrolysis and the Electrochemical Effects on Microorganisms' Population, Final Report, Septem - Novem   The Many Benefits of Hydrogen Peroxide [Editor's Note: Update The article seen below was posted 12 years ago.

At the time, I wanted to help spread the word that simple and inexpensive oxidative therapies, like hydrogen peroxide, could be used to address many serious health issues by intravenous application, or ingesting drops in a carefully controlled protocol, or inhaling the   Huling, S.

G., et al., "Enhanced Bioremediation Utilizing Peroxide as a Supplemental Source of Oxygen: A Laboratory and Field Study" Feb. Tabak H. H., et al., "Laboratory Studies Evaluating the Enhanced Biodegradation of Weathered Crude Oil Components through the Application of Nutrients", Jun.

A particulate material, as well as a method of manufacturing and using the material, for promoting growth of petroleum degrading bacteria to aid in bioremediation of oil spills on water and in wetlands which consists of a core of microbial available nutrients having a coating, comprised of oleic acid and either stearic acid, palmitic acid, or a mixture thereof, which is lipophilic, and   This chapter reviews the application of additives used in bioremediation of chlorinated solvents and fuels for groundwater and soil remediation.

Soluble carbon substrates are applicable to most site conditions except aquifers with very high or very low groundwater flow. Slow-release and solid substrates are intended to be long-lasting in supplying carbon for microbial growth thereby minimizing Hydrogen peroxide production is a well-known trait of many bacterial species associated with the human body.

In the presence of oxygen, the probiotic lactic acid bacterium Lactobacillus johnsonii NCC excretes up to 1 mM H2O2, inducing growth stagnation and cell death. Disruption of genes commonly assumed to be involved in H2O2 production (e.g., pyruvate oxidase, NADH oxidase, and lactate   Hydrogen peroxide was used as the oxygen source at a concentration of g/L nutrient solution.

The flask was then placed in the shake oven at rpm and 32° C. At the end of each week, one flask was removed and dried for analysis. Five mL of the mineral salt solution was added to the remaining flasks. This was repeated for eight Bioremediation uses microorganisms to degrade chemicals of interest and can be limited by mineral nutrients and terminal electron acceptors, especially oxygen.

This research investigated in situ bioremediation with hydrogen peroxide (H[sub 2]O[sub 2]) as a supplemental oxygen source, added in increasing concentration, and addressed the A particulate material, as well as a method of manufacturing and using the material, for promoting growth of petroleum degrading bacteria to aid in bioremediation of oil spills on water and in wetlands which consists of a core of microbial available nutrients having a coating, comprised of oleic or linoleic acid and either stearic acid, palmitic acid, or a mixture thereof, which is lipophilic [33] The hydrogen peroxide disproportionation rate constant k per has an estimated value of 22 days −1, which corresponds to a half‐life of 45 min.

This is within the range of ∼10– min found by Hinchee et al. in a field experiment where hydrogen peroxide was injected into an aquifer as an oxygen source for biodegradation of jet :// When hydrogen peroxide solutions are injected alone (i.e., without an iron catalyst), reductions in contaminant concentrations are frequently the result of volatilization or stripping, which occurs because of increased temperature and O 2 production as the hydrogen peroxide decomposes (Oberle 2 days ago  Soil Oxygen Soil Pollutants Reactive Oxygen Species Singlet Oxygen Soil Pollutants, Radioactive RNA, Ribosomal, 16S Oxygen Isotopes Fertilizers DNA, Ribosomal Nitrogen Carbon DNA, Bacterial Carbon Dioxide Water Hydrogen Peroxide Manure Metals, Heavy Humic Substances Antioxidants Nitrates Oxyhemoglobins Catalase Hemoglobins Free Radicals   Bioremediation uses microorganisms to degrade chemicals of interest and can be limited by mineral nutrients and terminal electron acceptors, especially oxygen.

This research investigated in situ bioremediation with hydrogen peroxide (H[sub 2]O[sub 2]) as a supplemental oxygen source, added in increasing concentration, and addressed the Biosurfactants are potentially replacements for synthetic surfactants in several industrial processes, such as lubrication, wetting, softening, fixing dyes, making emulsions, stabilizing dispersions, foaming, preventing foaming, as well as in food, biomedical and pharmaceutical industry, and bioremediation of organic- or inorganic-contaminated :// Micro-nano bubbles (MNBs) technology has shown great potential in groundwater bioremediation because of their large specific surface area, negatively charged surface, long stagnation, high oxygen transfer efficiency, etc.

Groundwater salinity, which varies from sites due to different geological and environmental conditions, has a strong impact on the bioremediation :// Enhanced Bioremediation of hydrocarbon contaminated mangrove swamp in the Nigerian oil-rich Niger Delta using seawater microbial inocula amended with crude biosurfactants and micronutrients.

Enhanced bioremediation of hydrocarbon-contaminated soil using pilot-scale bioelectrochemical :// Accumulating experimental evidence in mammalian, and recently plant, systems has led to a change in our understanding of the role played by hydrogen sulfide in life processes.

In plants, hydrogen sulfide mitigates stress and regulates important plant processes such as photosynthesis, stomatal movement, and autophagy, although the underlying mechanism is not well.

We previously reported that nitric oxide ([NO][1]) functions as a signal in thermotolerance. To illustrate its relationship with hydrogen peroxide ([H2O2][2]) in the tolerance of Arabidopsis (Arabidopsis thaliana) to heat shock ([HS][3]), we investigated the effects of heat on Arabidopsis seedlings of the following types: the wild type; three NADPH oxidase-defective mutants that exhibit Excavation, co-solvent flushing, and enhanced bioremediation with source control.

Site Description and Approach. Site Description: The soil profile at the site consists of silty, fine to very fine-grained sand with shell fragments from surface to 40–50 feet ://The best known and perhaps most common example is glucose.

Microbes can utilize hydrocarbons via a stepwise oxidation of a hydrocarbon by oxygen produces water and, successively, an alcohol, an aldehyde or a ketone, a carboxylic acid, and then a peroxide. Note the presence of oxygen, thus defining this as aerobic hydrocarbon ://:_Microbiology_(Boundless)/5.